Rapid communication: sequencing of the porcine agouti-related protein (AGRP) gene.

Name of Sequence. Porcine agouti-related protein (AGRP) gene. Genus and Species. Sus scrofa. Origin of Clone. Initially, a 978-bp fragment was amplified from genomic DNA using PCR primers (forward 5′-ATGCTGACCGCAGTGTTGCTGAGC-3′ and reverse 5′-CTAGGTGCGGCTGCAGGGGTTCGT-3′) designed from consensus alignment of human, bovine, and mouse DNA sequences. The PCR product was cloned into the pCR 2.1 vector (TOPO TA Cloning Kit, Invitrogen, Carlsbad, CA), and the cloned DNA fragment sent to the DNA Synthesis Facility, Iowa State University (Ames, IA), where a primer walking procedure was used to obtain the initial sequence. In order to extend the sequence in both directions, nested primers were designed from the known 978-bp sequence and used in combina-